Chemiluminescence dot blot hybridization assay for detection of B19 parvovirus DNA in human sera

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Chemiluminescence dot blot hybridization assay for detection of B19 parvovirus DNA in human sera.

A chemiluminescence dot blot hybridization assay was used for the detection of B19 parvovirus DNA in human sera by using digoxigenin-labeled probes. The probes were revealed immunoenzymatically by use of anti-digoxigenin Fab fragments conjugated with alkaline phosphatase. The chemiluminescence signal was obtained by reacting the labeled probe-target complex with an enzyme-triggerable dioxetane ...

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Dot blot hybridization assay of B19 virus DNA in clinical specimens.

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 1991

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.29.9.2047-2050.1991